Toxic chemical risk

Toxic chemical risk

2019L

Kod przedmiotu1849S2-TCR
Punkty ECTS 4
Typ zajęć Ćwiczenia komputerowe
Ćwiczenia laboratoryjne
Wykład
Przedmioty wprowadzającebiology, chemistry
Wymagania wstępnebasic knowledge of molecular genetics, good pippetting skills
Opis ćwiczeńHealth and Safety regulations, organizational issues, introduction to the subject. User manual of the molecular biology equipment. Pippetting micro volumes of liquids with different physical properties (density, viscosity). Lethal toxicity tests on fish. Calculation of median lethal concentration (LC50), median lethal time (LT50) values, and toxic units number. Phytotoxicity assessment using PHYTOTOX kit for tests on monocotyledonous (sorghum) and cotyledonous plants (cress, mustard). Physiological endpoints of toxicity: examination of blood smears and liver sections of fish exposed to polycyclic aromatic hydrocarbons (PAHs) using light microscopy (laboratory classess). Molecular toxicology: analysis of gene expression after exposure to model toxic substance. Genotoxicology: assessment of genotoxic effect of PAHs on fish’s erythrocytes and hepatocytes using the comet assay. Risk assessment: hazard identification, analysis of exposure, analysis of effects, rick characterization. Ecological risk assessment.
Opis wykładówToxic chemical risk as science. Environmental pathways of toxic chemicals. Dose effect. Toxicity testing in animals. Studies of human populations at risk. The body’s defenses against chemical toxicity. Mechanisms of chemical disease. Human health risk assessment. Ecological risk assessment. Managing chemical risk
Cel kształceniaTo make students familiar with the science that underlies toxic chemical risk analysis, the physiological and molecular basis of chemical toxicity, the process of assessing toxic chemical risk to human health and environment, and the strategies employed in managing it
Literatura podstawowa1) Logan, J., Edwards, K., Saunders, N., Real Time PCR: Current technology and applications. , Academic Press,, 2009 2) Vanden Heuvel, J.P.,, Protocols in Molecular Toxicology, , CRC Press, , 1998 3) Fisher J., Arnold, J.R.P.,, Chemistry for Biologists. Instant Notes Series., Bios Scientific Publishers, Oxford, , 2000 4) Brown, T.A. , Molecular Biology Labfax., BIOS Scientific Publishers, Oxford,UK, , 1991
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